bacillus halodurans atcc baa 125  (ATCC)

Journal: Applied Microbiology and Biotechnology

Article Title: L-rhamnose isomerase: a crucial enzyme for rhamnose catabolism and conversion of rare sugars

doi: 10.1007/s00253-024-13325-w

Figure Lengend Snippet: Comparison of the enzyme properties of various L-RhIs

Article Snippet: Bacillus halodurans ATCC BAA-125 , 121/48, homodimer homotetramer* , Halalkalibacterium halodurans ATCC BAA-125 (BAB05271.1), 418 a.a., 48,178 Da , 7.0 , 70 , Mn 2+ , 528 , 0.28 , 187 , L-lyxose > , L-mannose, D-gulose, L-talose , Prabhu et al. Prabhu et al. * .

Techniques: Comparison, Sequencing

Journal: PLOS Biology

Article Title: Archaea produce peptidoglycan hydrolases that kill bacteria

doi: 10.1371/journal.pbio.3003235

Figure Lengend Snippet: A. Schematic overview of the pipeline for identifying bacteria targeted by peptidoglycan hydrolases. The cell wall/peptidoglycan binding domain of a putative archaeal peptidoglycan hydrolase (PGH) is searched against a database of archaeal and bacterial PGH homologs, using either primary sequence (HMMer) or structural homology (Foldseek) searches. Domain matches are ranked by similarity, normalized to the score obtained by matching against the query protein itself. B. The cell wall binding domains of phage endolysins are typically most similar to the cell wall binding domains of their (predicted) bacterial hosts compared to domains from other bacteria. C. Probability that, for a given archaeal query protein, bacteria from the same biome are identified amongst the top 10 hits. Biome labels were applied as described in and results are aggregated at the biome level; the size of each circle indicates the likelihood ratio associated with a given biome. D. Ranking of homologous bacterial hits against the PG binding 1 domain of Woldo based on sequence vs. structural homology scores (Spearman’s rho = 0.48). E. Enrichment of bacterial phyla amongst top 10 predicted targets across PGH homologs. See for how enrichment was computed F. Results of spotting various supernatants onto lawns of Halalkalibacterium halodurans , Phycicoccus endophyticus , and Virgibacillus salexigens . a,b: top fraction (>3 kDa) of supernatant from Haloferax volcanii expressing an intact (a) or catalytic mutant (b) version of Woldo; c,d: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing an intact (c) or catalytic mutant (d) version of Woldo; e: unfiltered supernatant from Halogranum salarium B-1; f,g: top fraction (>3 kDa) of supernatant from H. volcanii expressing (f) Danwoldo or (g) and empty plasmid control; h,i: bottom fraction (<3 kDa) of supernatant from H. volcanii expressing (h) Danwoldo or (i) and empty plasmid control. G. Colony forming assays for different bacteria following treatment with supernatant from H. volcanii expressing Woldo, Danwoldo, or the control (bottom fraction of Woldo-expressing H. volcanii H1424 supernatant). Error bars represent standard deviation from three biological replicates. H. Images of bacterial cells following exposure to H. volcanii supernatants expressing Woldo, Danwoldo or the control (as above). Bacteria are stained using LIVE/DEAD BacLight bacterial viability staining kit with ingress of red dye into bacterial cells indicative of cells with terminally compromised cell wall integrity. Code and data are available at zenodo.org/records/15534318 (“notebook/figure3.ipynb” and “data/figure3/”, respectively).

Article Snippet: Halogranum salarium B-1 (DSM-23171), Halalkalibacterium halodurans (DSM-18197), Phycicoccus endophyticus (DSM-100020), and Virgibacillus salexigens (DSM-11483) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ).

Techniques: Bacteria, Binding Assay, Sequencing, Expressing, Mutagenesis, Plasmid Preparation, Control, Standard Deviation, Staining

Journal: Microorganisms

Article Title: Bacterial Viability in Self-Healing Concrete: A Case Study of Non-Ureolytic Bacillus Species

doi: 10.3390/microorganisms11102402

Figure Lengend Snippet: Microscopic images (ranging in magnification from 24× to 28×) of mortar samples with a crack width of 0.130-0.136 mm before and after 7 and 35 days of healing using microcapsules with different bacterial species: ( a ) B. pseudofirmus ; ( b ) B. cohnii ; ( c ) B. halodurans. Here, w i is the initial crack width; w t is the crack width after healing; H c is the calculated healing ratio. The blue arrows indicate the location where the width of the gap was measured.

Article Snippet: Three alkaliphilic and spore-forming bacteria species, i.e., B. pseudofirmus (DSM 8715), B. cohnii (DSM 6307), and B. halodurans (DSM 497), were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ), Braunschweig, Germany.

Techniques:

Journal: Microorganisms

Article Title: Bacterial Viability in Self-Healing Concrete: A Case Study of Non-Ureolytic Bacillus Species

doi: 10.3390/microorganisms11102402

Figure Lengend Snippet: Microscopic images (ranging in magnification from 24× to 28×) of mortar samples with a crack width of 0.213-0.288 mm before and after 14 and 35 days of healing using microcapsules with different bacterial species: ( a ) B. pseudofirmus ; ( b ) B. cohnii ; ( c ) B . halodurans. Here, w i is the initial crack width; w t is the crack width after healing; H c is the calculated healing ratio. The blue arrows indicate the location where the width of the gap was measured.

Article Snippet: Three alkaliphilic and spore-forming bacteria species, i.e., B. pseudofirmus (DSM 8715), B. cohnii (DSM 6307), and B. halodurans (DSM 497), were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ), Braunschweig, Germany.

Techniques: